Bacillus megaterium where is it found

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Laemmli UK. Cleavage of structural proteins during assembly of head of bacteriophage T4. Download references. All authors read and approved the final manuscript. The authors would like to thank Dr. Rebekka Biedendieck for plasmids and technical advice, Dr. Jason Lee for help with testing the A5 medium, and Dr. Iain Hay for advice and experimental assistance. The authors are also grateful to the Manawatu Microscopy and Imaging Centre for technical assistance in electron microscopy.

The datasets generated and analysed during the current study are available from the corresponding author on reasonable request. Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Bioline Reagents Ltd. You can also search for this author in PubMed Google Scholar.

Correspondence to Bernd H. Reprints and Permissions. Grage, K. Engineering Bacillus megaterium for production of functional intracellular materials. Microb Cell Fact 16, Download citation. Received : 24 August Accepted : 13 November Published : 22 November Anyone you share the following link with will be able to read this content:.

Sorry, a shareable link is not currently available for this article. Provided by the Springer Nature SharedIt content-sharing initiative. Skip to main content. Search all BMC articles Search. Download PDF. Abstract Background Over the last 10—15 years, a technology has been developed to engineer bacterial poly 3-hydroxybutyrate PHB inclusions as functionalized beads, for applications such as vaccines, diagnostics and enzyme immobilization. Results In this study, we have bioengineered the formation of functional PHB inclusions in the Gram-positive bacterium Bacillus megaterium , an LPS-free and established industrial production host.

Conclusions This study provides proof of concept for the successful genetic engineering of B. Results Bioengineering of B. Optimization of growth medium, growth and induction conditions In recombinant E.

Full size image. Table 1 Sporulation assays Full size table. Discussion In this study, we have explored the possible use of the Gram-positive endotoxin-free host B. Conclusions This study demonstrates the successful genetic engineering of B. Table 2 Strains and plasmids used in this study Full size table. References 1. Article Google Scholar 4.

Article Google Scholar 7. Article Google Scholar CAS Google Scholar Google Scholar Chapter Google Scholar Acknowledgements The authors would like to thank Dr. Availability of data and materials The datasets generated and analysed during the current study are available from the corresponding author on reasonable request. Consent for publication Not applicable.

Ethics approval and consent to participate Not applicable. Funding This study was funded by PolyBatics Ltd. Rehm Authors Katrin Grage View author publications. View author publications. Bacillus megaterium is a good source of industrial proteins because it is both a desirable cloning host and produces a large variation of enzymes. This species is good cloning host because it is able to house numerous plasmid vectors while remaining stable due to its unique external proteases.

The organism does not have alkaline proteases; which allows for recombinant protein synthesis. Using Bacillus megaterium scientist have developed numerous proteins that are commonly used in the medical and agricultural field. Chen, L.

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